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fibrillar type i collagen (horm)  (Takeda)

 
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    Takeda fibrillar type i collagen (horm)
    (a-c) Washed platelets were incubated in vitro with 10 μg/ml of the indicated antibodies for 5 min and stimulated with <t>Horm</t> or soluble collagen. Light transmission was recorded on an Apact four-channel aggregometer over 20 min. Representative aggregation curves of n=3, 2 independent experiments. LEN/B: anti-α2 integrin antibody51. (d, e) Flow cytometry reveals unaltered reactivity of DOM/B-treated WT platelets upon thrombin stimulation compared to WT controls. Mean ± SD. n=3 mice, 4 independent experiments, two-tailed unpaired t-test with Welch’s correction. (f) Aggregation upon thrombin-stimulation is not affected in the presence of DOM/B. Light transmission was recorded on an Apact four-channel aggregometer over 10 min. Representative curves of n=3, 3 individual experiments. (g) Thrombin exosites I and II were blocked by the aptamers HD1 and HD22, respectively. Platelets were incubated with the indicated antibody (10 μg/ml) prior to thrombin stimulation. Thrombin-mediated cleavage of GPV was assessed by flow cytometry. Mean ± SD. n=3 mice, 3 independent experiments. (h) Thrombin clotting time was assessed using a ball coagulometer in GPV mutant or anti-mGPV mAb treated PRP in the presence or absence of HD22. Antibody concentration: 10 μg/ml; aptamer concentration: 1.5 μM f.c., thrombin: 17 nM. Mean ± SD. WT, WT+DOM/B, WT+DOM/C: n=5, Gp5−/−: n=4, Gp5dThr, Gp5−/−+HD22, Gp5dThr+HD22: n=3, WT+DOM/B+HD22, WT+DOM/C+HD22: n=6, WT+HD22: n=8. One-way ANOVA followed by Dunn’s test for multiple comparisons.
    Fibrillar Type I Collagen (Horm), supplied by Takeda, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fibrillar+type+i+collagen+horm/fibrillar+type+i+collagen/pmc10195106-500-0-7
    Average 90 stars, based on 1 article reviews
    fibrillar type i collagen (horm) - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Platelet glycoprotein V spatio-temporally controls fibrin formation"

    Article Title: Platelet glycoprotein V spatio-temporally controls fibrin formation

    Journal: Nature cardiovascular research

    doi: 10.1038/s44161-023-00254-6

    (a-c) Washed platelets were incubated in vitro with 10 μg/ml of the indicated antibodies for 5 min and stimulated with Horm or soluble collagen. Light transmission was recorded on an Apact four-channel aggregometer over 20 min. Representative aggregation curves of n=3, 2 independent experiments. LEN/B: anti-α2 integrin antibody51. (d, e) Flow cytometry reveals unaltered reactivity of DOM/B-treated WT platelets upon thrombin stimulation compared to WT controls. Mean ± SD. n=3 mice, 4 independent experiments, two-tailed unpaired t-test with Welch’s correction. (f) Aggregation upon thrombin-stimulation is not affected in the presence of DOM/B. Light transmission was recorded on an Apact four-channel aggregometer over 10 min. Representative curves of n=3, 3 individual experiments. (g) Thrombin exosites I and II were blocked by the aptamers HD1 and HD22, respectively. Platelets were incubated with the indicated antibody (10 μg/ml) prior to thrombin stimulation. Thrombin-mediated cleavage of GPV was assessed by flow cytometry. Mean ± SD. n=3 mice, 3 independent experiments. (h) Thrombin clotting time was assessed using a ball coagulometer in GPV mutant or anti-mGPV mAb treated PRP in the presence or absence of HD22. Antibody concentration: 10 μg/ml; aptamer concentration: 1.5 μM f.c., thrombin: 17 nM. Mean ± SD. WT, WT+DOM/B, WT+DOM/C: n=5, Gp5−/−: n=4, Gp5dThr, Gp5−/−+HD22, Gp5dThr+HD22: n=3, WT+DOM/B+HD22, WT+DOM/C+HD22: n=6, WT+HD22: n=8. One-way ANOVA followed by Dunn’s test for multiple comparisons.
    Figure Legend Snippet: (a-c) Washed platelets were incubated in vitro with 10 μg/ml of the indicated antibodies for 5 min and stimulated with Horm or soluble collagen. Light transmission was recorded on an Apact four-channel aggregometer over 20 min. Representative aggregation curves of n=3, 2 independent experiments. LEN/B: anti-α2 integrin antibody51. (d, e) Flow cytometry reveals unaltered reactivity of DOM/B-treated WT platelets upon thrombin stimulation compared to WT controls. Mean ± SD. n=3 mice, 4 independent experiments, two-tailed unpaired t-test with Welch’s correction. (f) Aggregation upon thrombin-stimulation is not affected in the presence of DOM/B. Light transmission was recorded on an Apact four-channel aggregometer over 10 min. Representative curves of n=3, 3 individual experiments. (g) Thrombin exosites I and II were blocked by the aptamers HD1 and HD22, respectively. Platelets were incubated with the indicated antibody (10 μg/ml) prior to thrombin stimulation. Thrombin-mediated cleavage of GPV was assessed by flow cytometry. Mean ± SD. n=3 mice, 3 independent experiments. (h) Thrombin clotting time was assessed using a ball coagulometer in GPV mutant or anti-mGPV mAb treated PRP in the presence or absence of HD22. Antibody concentration: 10 μg/ml; aptamer concentration: 1.5 μM f.c., thrombin: 17 nM. Mean ± SD. WT, WT+DOM/B, WT+DOM/C: n=5, Gp5−/−: n=4, Gp5dThr, Gp5−/−+HD22, Gp5dThr+HD22: n=3, WT+DOM/B+HD22, WT+DOM/C+HD22: n=6, WT+HD22: n=8. One-way ANOVA followed by Dunn’s test for multiple comparisons.

    Techniques Used: In Vitro, Incubation, Transmission Assay, Flow Cytometry, Two Tailed Test, Coagulation, Mutagenesis, Concentration Assay

    Related Articles

    other:

    Article Title: Functionalized polymer microbubbles as new molecular ultrasound contrast agent to target P-selectin in thrombus.
    Article Snippet: Fibrillar type I collagen Horm® was obtained from Takeda (Linz, Austria).

    Article Title: Thrombolytic therapy based on fucoidan-functionalized polymer nanoparticles targeting P-selectin.
    Article Snippet: 5 6 Flow binding assay of nanoparticles on activated platelet aggregates 7 To further investigate the binding efficiency of unloaded and loaded NPs to activated platelets, 8 channels of Vena8 Fluoro+ chambers were coated with 50 μg/mL of fibrillar type I collagen 9 Horm® (Takeda, Linz, Austria) overnight and rinsed with NaCl 0.9% before use.

    Article Title: Platelet glycoprotein V spatio-temporally controls fibrin formation.
    Article Snippet: Fibrillar type I collagen (Horm) was from Takeda; rhodocytin was provided by J. Eble (University of Münster, Münster, Germany).



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    (a-c) Washed platelets were incubated in vitro with 10 μg/ml of the indicated antibodies for 5 min and stimulated with <t>Horm</t> or soluble collagen. Light transmission was recorded on an Apact four-channel aggregometer over 20 min. Representative aggregation curves of n=3, 2 independent experiments. LEN/B: anti-α2 integrin antibody51. (d, e) Flow cytometry reveals unaltered reactivity of DOM/B-treated WT platelets upon thrombin stimulation compared to WT controls. Mean ± SD. n=3 mice, 4 independent experiments, two-tailed unpaired t-test with Welch’s correction. (f) Aggregation upon thrombin-stimulation is not affected in the presence of DOM/B. Light transmission was recorded on an Apact four-channel aggregometer over 10 min. Representative curves of n=3, 3 individual experiments. (g) Thrombin exosites I and II were blocked by the aptamers HD1 and HD22, respectively. Platelets were incubated with the indicated antibody (10 μg/ml) prior to thrombin stimulation. Thrombin-mediated cleavage of GPV was assessed by flow cytometry. Mean ± SD. n=3 mice, 3 independent experiments. (h) Thrombin clotting time was assessed using a ball coagulometer in GPV mutant or anti-mGPV mAb treated PRP in the presence or absence of HD22. Antibody concentration: 10 μg/ml; aptamer concentration: 1.5 μM f.c., thrombin: 17 nM. Mean ± SD. WT, WT+DOM/B, WT+DOM/C: n=5, Gp5−/−: n=4, Gp5dThr, Gp5−/−+HD22, Gp5dThr+HD22: n=3, WT+DOM/B+HD22, WT+DOM/C+HD22: n=6, WT+HD22: n=8. One-way ANOVA followed by Dunn’s test for multiple comparisons.
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    (a-c) Washed platelets were incubated in vitro with 10 μg/ml of the indicated antibodies for 5 min and stimulated with <t>Horm</t> or soluble collagen. Light transmission was recorded on an Apact four-channel aggregometer over 20 min. Representative aggregation curves of n=3, 2 independent experiments. LEN/B: anti-α2 integrin antibody51. (d, e) Flow cytometry reveals unaltered reactivity of DOM/B-treated WT platelets upon thrombin stimulation compared to WT controls. Mean ± SD. n=3 mice, 4 independent experiments, two-tailed unpaired t-test with Welch’s correction. (f) Aggregation upon thrombin-stimulation is not affected in the presence of DOM/B. Light transmission was recorded on an Apact four-channel aggregometer over 10 min. Representative curves of n=3, 3 individual experiments. (g) Thrombin exosites I and II were blocked by the aptamers HD1 and HD22, respectively. Platelets were incubated with the indicated antibody (10 μg/ml) prior to thrombin stimulation. Thrombin-mediated cleavage of GPV was assessed by flow cytometry. Mean ± SD. n=3 mice, 3 independent experiments. (h) Thrombin clotting time was assessed using a ball coagulometer in GPV mutant or anti-mGPV mAb treated PRP in the presence or absence of HD22. Antibody concentration: 10 μg/ml; aptamer concentration: 1.5 μM f.c., thrombin: 17 nM. Mean ± SD. WT, WT+DOM/B, WT+DOM/C: n=5, Gp5−/−: n=4, Gp5dThr, Gp5−/−+HD22, Gp5dThr+HD22: n=3, WT+DOM/B+HD22, WT+DOM/C+HD22: n=6, WT+HD22: n=8. One-way ANOVA followed by Dunn’s test for multiple comparisons.
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    (a-c) Washed platelets were incubated in vitro with 10 μg/ml of the indicated antibodies for 5 min and stimulated with <t>Horm</t> or soluble collagen. Light transmission was recorded on an Apact four-channel aggregometer over 20 min. Representative aggregation curves of n=3, 2 independent experiments. LEN/B: anti-α2 integrin antibody51. (d, e) Flow cytometry reveals unaltered reactivity of DOM/B-treated WT platelets upon thrombin stimulation compared to WT controls. Mean ± SD. n=3 mice, 4 independent experiments, two-tailed unpaired t-test with Welch’s correction. (f) Aggregation upon thrombin-stimulation is not affected in the presence of DOM/B. Light transmission was recorded on an Apact four-channel aggregometer over 10 min. Representative curves of n=3, 3 individual experiments. (g) Thrombin exosites I and II were blocked by the aptamers HD1 and HD22, respectively. Platelets were incubated with the indicated antibody (10 μg/ml) prior to thrombin stimulation. Thrombin-mediated cleavage of GPV was assessed by flow cytometry. Mean ± SD. n=3 mice, 3 independent experiments. (h) Thrombin clotting time was assessed using a ball coagulometer in GPV mutant or anti-mGPV mAb treated PRP in the presence or absence of HD22. Antibody concentration: 10 μg/ml; aptamer concentration: 1.5 μM f.c., thrombin: 17 nM. Mean ± SD. WT, WT+DOM/B, WT+DOM/C: n=5, Gp5−/−: n=4, Gp5dThr, Gp5−/−+HD22, Gp5dThr+HD22: n=3, WT+DOM/B+HD22, WT+DOM/C+HD22: n=6, WT+HD22: n=8. One-way ANOVA followed by Dunn’s test for multiple comparisons.
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    (a-c) Washed platelets were incubated in vitro with 10 μg/ml of the indicated antibodies for 5 min and stimulated with <t>Horm</t> or soluble collagen. Light transmission was recorded on an Apact four-channel aggregometer over 20 min. Representative aggregation curves of n=3, 2 independent experiments. LEN/B: anti-α2 integrin antibody51. (d, e) Flow cytometry reveals unaltered reactivity of DOM/B-treated WT platelets upon thrombin stimulation compared to WT controls. Mean ± SD. n=3 mice, 4 independent experiments, two-tailed unpaired t-test with Welch’s correction. (f) Aggregation upon thrombin-stimulation is not affected in the presence of DOM/B. Light transmission was recorded on an Apact four-channel aggregometer over 10 min. Representative curves of n=3, 3 individual experiments. (g) Thrombin exosites I and II were blocked by the aptamers HD1 and HD22, respectively. Platelets were incubated with the indicated antibody (10 μg/ml) prior to thrombin stimulation. Thrombin-mediated cleavage of GPV was assessed by flow cytometry. Mean ± SD. n=3 mice, 3 independent experiments. (h) Thrombin clotting time was assessed using a ball coagulometer in GPV mutant or anti-mGPV mAb treated PRP in the presence or absence of HD22. Antibody concentration: 10 μg/ml; aptamer concentration: 1.5 μM f.c., thrombin: 17 nM. Mean ± SD. WT, WT+DOM/B, WT+DOM/C: n=5, Gp5−/−: n=4, Gp5dThr, Gp5−/−+HD22, Gp5dThr+HD22: n=3, WT+DOM/B+HD22, WT+DOM/C+HD22: n=6, WT+HD22: n=8. One-way ANOVA followed by Dunn’s test for multiple comparisons.
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    (a-c) Washed platelets were incubated in vitro with 10 μg/ml of the indicated antibodies for 5 min and stimulated with Horm or soluble collagen. Light transmission was recorded on an Apact four-channel aggregometer over 20 min. Representative aggregation curves of n=3, 2 independent experiments. LEN/B: anti-α2 integrin antibody51. (d, e) Flow cytometry reveals unaltered reactivity of DOM/B-treated WT platelets upon thrombin stimulation compared to WT controls. Mean ± SD. n=3 mice, 4 independent experiments, two-tailed unpaired t-test with Welch’s correction. (f) Aggregation upon thrombin-stimulation is not affected in the presence of DOM/B. Light transmission was recorded on an Apact four-channel aggregometer over 10 min. Representative curves of n=3, 3 individual experiments. (g) Thrombin exosites I and II were blocked by the aptamers HD1 and HD22, respectively. Platelets were incubated with the indicated antibody (10 μg/ml) prior to thrombin stimulation. Thrombin-mediated cleavage of GPV was assessed by flow cytometry. Mean ± SD. n=3 mice, 3 independent experiments. (h) Thrombin clotting time was assessed using a ball coagulometer in GPV mutant or anti-mGPV mAb treated PRP in the presence or absence of HD22. Antibody concentration: 10 μg/ml; aptamer concentration: 1.5 μM f.c., thrombin: 17 nM. Mean ± SD. WT, WT+DOM/B, WT+DOM/C: n=5, Gp5−/−: n=4, Gp5dThr, Gp5−/−+HD22, Gp5dThr+HD22: n=3, WT+DOM/B+HD22, WT+DOM/C+HD22: n=6, WT+HD22: n=8. One-way ANOVA followed by Dunn’s test for multiple comparisons.

    Journal: Nature cardiovascular research

    Article Title: Platelet glycoprotein V spatio-temporally controls fibrin formation

    doi: 10.1038/s44161-023-00254-6

    Figure Lengend Snippet: (a-c) Washed platelets were incubated in vitro with 10 μg/ml of the indicated antibodies for 5 min and stimulated with Horm or soluble collagen. Light transmission was recorded on an Apact four-channel aggregometer over 20 min. Representative aggregation curves of n=3, 2 independent experiments. LEN/B: anti-α2 integrin antibody51. (d, e) Flow cytometry reveals unaltered reactivity of DOM/B-treated WT platelets upon thrombin stimulation compared to WT controls. Mean ± SD. n=3 mice, 4 independent experiments, two-tailed unpaired t-test with Welch’s correction. (f) Aggregation upon thrombin-stimulation is not affected in the presence of DOM/B. Light transmission was recorded on an Apact four-channel aggregometer over 10 min. Representative curves of n=3, 3 individual experiments. (g) Thrombin exosites I and II were blocked by the aptamers HD1 and HD22, respectively. Platelets were incubated with the indicated antibody (10 μg/ml) prior to thrombin stimulation. Thrombin-mediated cleavage of GPV was assessed by flow cytometry. Mean ± SD. n=3 mice, 3 independent experiments. (h) Thrombin clotting time was assessed using a ball coagulometer in GPV mutant or anti-mGPV mAb treated PRP in the presence or absence of HD22. Antibody concentration: 10 μg/ml; aptamer concentration: 1.5 μM f.c., thrombin: 17 nM. Mean ± SD. WT, WT+DOM/B, WT+DOM/C: n=5, Gp5−/−: n=4, Gp5dThr, Gp5−/−+HD22, Gp5dThr+HD22: n=3, WT+DOM/B+HD22, WT+DOM/C+HD22: n=6, WT+HD22: n=8. One-way ANOVA followed by Dunn’s test for multiple comparisons.

    Article Snippet: Fibrillar type I collagen (Horm) was from Takeda, Rhodocytin was provided by Johannes Eble (University of Münster, Münster, Germany).

    Techniques: In Vitro, Incubation, Transmission Assay, Flow Cytometry, Two Tailed Test, Coagulation, Mutagenesis, Concentration Assay